GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues.
DyLight™ 594 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight™ 594 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.
Pseudorabies virus hijacks DDX3X, initiating an addictive “mad itch” and immune suppression, to facilitate viral spread Cronin Shane J. F., Tejada Miguel A., ..., Penninger Josef M.
Article Snippet "PrePrint: .. 3 times in PBS (10 minutes each) and mounted using Dako mounting medium (S3023).. The following primary antibodies were used: Isolectin B4 (IB4) DyLight 594-conjugated (Vector laboratories FL- 1207); CGRP (Millipore; PC205L), DDX3X (santa cruz; sc-365768); NF200 (Abcam; ab4680); Parvalbumin (Swant; PV-25).. Secondary antibodies used: Alexa Fluor 555 anti-rabbit (Invitrogen; A32794), Alexa Fluor 555 anti-mouse (Invitrogen; ..." More Figure Legend "... tamoxifen or vehicle (oil) stained with anti-CGRP and IB4-lectin ( f ) and quantification of the number ..." More