大家都在搜
手机验证
我的询价
询价列表
暂时没有已询价产品
ADVANCEDtm qPCR PROBE mastermix
wisent
100 reactions
| CAT.NO | DESCRIPTION | REMARKS | SIZE |
| 801-071-AR | Compatible on all real-time PCR platforms and various
| LOW ROX
| 100 reactions |
| 801-071-ER | 500 reactions | ||
| 801-071-QR | 2000 reactions | ||
| 801-072-AR | HIGH ROX
| 100 reactions | |
| 801-072-ER | 500 reactions | ||
| 801-072-QR | 2000 reactions | ||
| 801-073-AR | NO ROX
| 100 reactions | |
| 801-073-ER | 500 reactions | ||
| 801-073-QR | 2000 reactions |
Product Description:
WISENT ADVANCED PROBEmastermix has following advantagers:
· High efficiency in multiplex reactions
· Rapid extension rate for early Ct values
· Market leading sensitivity - increased limit of detection
· Compatible on all real-time PCR platforms - standard and fast cycling conditions
Wisent Probe Mix is a universal probe kit designed for use in all probe based real-time PCR assays. Whether your application is for a singleplex or multiplex expression study, a genotyping experiment or a diagnostic assay,Wisent Probe Mix is the best choice for all your probe based real-time PCR needs.Wisent Probe Mix can be used to quantify DNA templats wether genomic, cDNA and viral sequences. Extremely low copy number targets can be detected with high efficiency. Optimized for use in fast and ultra fast cycling conditions.
Product Stability And Storage:
On arrival store at -20°C.
Avoid prolonged exposure to light.
If stored correctly the mastermix will retain full activity for 12 months. It is stable at 4°C for 1 month and can go through 30 freeze/thaw cycles with no loss of activity.
Instructions For Use:
Note: 1 For efficient amplification under fast cycling conditions we recommend amplicon lengths between 80bp and 200bp. Do not exceed 400 bp. The shorter the amplicon length the faster the reaction can be cycled. Primers should have a predicted melting temperature of around 60°C, using default Primer 3 settings. Note : 2 For TAQMANR probes choose probe close to 5’ primer and avoid terminal guanosine residues.
Reaction Setup:
1 Before starting, briefly vortex 2x WISENT ADVANCED mix .
2 Prepare a final mix , recommended20 ul, as follows:
| Add | Volume | Final concentration |
| WISENT ADVANCED mix | 10 ul | 1X |
| Forward primer (10 uM) | 0.8 ul | 400 nM |
| Reverse primer (10 uM) | 0.8 ul | 400 nM |
| Probe | 0.4ul | 200nM |
| Template DNA | cDNA :< 100 ng | variable |
| Add PCR grade water up to 20 ul final volume | ||
3 Program your instrument using following conditions, acquiring data on the SYBR® Green or FAM channel:
| Cycles | Temperature | Time | Notes |
| 1 | 95°C | 2min: cDNA | Polymerase activation |
| 40 | 95°C | 5 seconds | Denaturation |
| 60°C to 65°C | 20-30 seconds | Anneal/Extension do not exceed 30 seconds ; do not use bellow 60 °C |
FAM channel:
APPENDIX I
HIGH ROX / LOW ROX INSTRUMENT COMPATIBILITY
FOR FOLLOWING KITS:
-WISENT ADVANCEDtm qPCR MASTERMIX WITH SUPERGREENtm DYE
-WISENT ADVANCEDtm qPCR 1 step mastermix with SUPERGREENtm dye
-WISENT ADVANCEDtm qPCR PROBE 1 step mastermix
-WISENT ADVANCEDtm qPCR GENOTYPING mastermix
| Manufacturer | Instrument | Lo-ROX | Hi ROX |
| Analytica Jena | qTower | Yes | Yes |
| Applied Biosystems | 7500, 7500 FAST, Viia7TM | Yes | No |
| Applied Biosystems | 7000, 7300,7700,7900, 7900HT, 7900HT FAST, StepOneTM, StepOneTM Plus | No | Yes |
| Bio-Rad® | iCycler®, MyiQ®, iQ TM5, OpticonTM, OpticonTM2, Chromo4TM, MiniOpticonTM, CFX96TM, CFX384TM | Yes | No |
| Cepheid® | Smartcycler® | Yes | Yes |
| Eppendorf | Mastercycler® ep realplex, Mastercycler® realplex 2S | Yes | Yes |
| Illumina® | EcoTM | Yes | Yes |
| Qiagen/Corbett | Rotor-GeneTM 3000, 6000, Q | Yes | Yes |
| Roche Applied Science | Lightcycler®480, Lightcycler®Nano | Yes | Yes |
| Stratagene (Agilent) | MX 4000P®, MX 3000P®, MX 3005P® | Yes | No |
| Takara | Cycler Dice® | Yes | Yes |
| Techne | Quantica® | Yes | Yes |
| Manufacturer | Instrument | Lo-ROX | Hi ROX |
Note: when offered No-ROX is compatible with all machines above
热线：400-823-3718
网址：www.wisentbioproductschina.com
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
该产品暂未上传技术资料
免费索取技术资料刘先生
南京市鼓楼区建宁路65号金川科技园14栋
细胞库 / 细胞培养,试剂
生产厂商
2023.03.21-2023.06.30
2023.03.21-2023.06.30
2023.03.08-2023.04.30
