精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Rat Angiopoietin 2,ANG-2 ELISA Kit
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 89
Range % 86-92
1:2 Average % 104
Range % 98-106
1:4 Average % 92
Range % 88-95
1:8 Average % 99
Range % 95-103
Rat Angiopoietin 2,ANG-2 ELISA Kit
回收率:
The recovery of rat ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 89-97
EDTA plasma (n=4) 97 94-99