ScienCell's GeneQuery™ human neural plasticity qPCR array kit (GQH-NPL) is designed to facilitate gene expression profiling of key genes involved in human synaptic plasticity, and related diseases, such as ADHD, autism, Down syndrome, and schizophrenia. 88 genes are selected in this kit based on public database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Neural Plasticity qPCR Array Plate Layout* (8 controls in Bold and Italic)
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A
ADAM10
CDH2
DISC2
FBN1
GRIA4
HTR2A
MAPK1
NLGN4X
PCDH8
PPP2CA
RHEB
β-actin
B
ADCY1
CEBPB
DSCAM
FGFR2
GRIN2B
IGF1
MECP2
NOS1
PICK1
PRKCA
RPL10
GAPDH
C
ADCY8
CEBPD
DSCR3
FOS
GRIN2D
INHBA
MMP9
NPTX2
PIGP
PRKG1
SLC6A2
LDHA
D
ARC
CNR1
EGR1
GABRA5
GRIP1
JUN
NFKB1
NR4A1
PIM1
RAB3A
SLC6A4
NONO
E
AUTS2
CNTNAP2
EGR2
GABRB3
GRM2
JUNB
NFKBIB
NRG1
PLAT
RELA
SRF
PPIH
F
BDNF
COMT
EGR4
GNAI1
GRM3
KCNQ1
NGF
NTF3
PLCG1
RELN
SYNPO
GDC
G
CAMK2A
CREB1
EN2
GRIA1
HOMER1
KLF10
NGFR
NTF4
PPP1CA
RGS2
TNF
PPC
H
CAMK2G
DAOA
EPHB2
GRIA2
HTR1B
MAOA
NLGN3
NTRK2
PPP1CC
RGS4
YWHAQ
NTC
* gene selection may be updated based on new research and development