ScienCell's GeneQuery™ human pluripotent stem cell qPCR array kit (GQH-hPSC) is designed to facilitate gene expression profiling of key genes involved in maintaining pluripotency and self-renewal of hPSC, and genes regulated during differentiation of hPSC. 88 genes are selected in this kit based on public database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Pluripotent Stem Cell qPCR Array Plate Layout* (8 controls in Bold and Italic)
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A
ACTC1
CDX2
EN2
FOXA2
GFAP
ISL1
MIXL1
NOG
PECAM1
SOX1
TBX6
β-actin
B
AFP
CER1
ENO2
FOXD3
GJA1
KLF4
MYF5
NPM1
POU5F1
SOX2
TCF2
GAPDH
C
ALB
COL1A1
EOMES
FOXJ3
GSC
LEFTYA
MYOD1
NR5A2
PTF1A
SOX7
TCF3
LDHA
D
ALDH1A1
COL2A1
ESG1
GATA2
HAND1
LEFTYB
NANOG
OLIG2
REST
SOX17
TDGF1
NONO
E
BRIX1
CXCR4
ESRRB
GATA4
HDAC2
LIN28
NEUROD
OTX2
REX1
T
TERT
PPIH
F
CD34
DES
FGF4
GATA6
HLXB9
MEF2C
NCAM1
PAX2
RUNX1
TAT
TUBB3
GDC
G
CD90
DNMT3B
FGF5
GBX2
HNF4A
MEIS1
NES
PAX6
RUNX2
TBX3
UTF1
PPC
H
CDH1
EMX2
FLT1
GDF3
IDH1
MESP1
NKX2.5
PDX-1
SALL4
TBX5
VIM
NTC
* gene selection may be updated based on new research and development