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Recombinant Streptavidin

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产品详情

品牌: BBI
货号: C600432
供应商: BBI
规格: 1 mg
规格编号:C600432-0001
概述:Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.
使用说明:This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Purity > 98 % by SDS-PAGE and HPLC analyses
Source Recombinant streptavidin from Streptomyces avidinii, produced in Escherichia coli.
Specific Activity > 17 U/mg (one unit binds 1 μg D-biotin at pH 8.9)
Formulation Lyophilized in 10 mM potassium phosphate buffer pH 6.5.
Endotoxin Less than 0.1 EU/μg of rStreptavidin as determined by LAL method.
Reconstitution Dissolve with double distilled water
AA Sequence MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS
Shipment At ambient temperature
A282 of 0.1% solution 3.1
Proteolytic Activity < 10-3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0)
Solubility Clearly soluble in ddH2O (OD405nm < 0.1 at 10 mg/ml water)
Stability (lyophilized) 2 years after shipping
温馨提示:不可用于临床治疗。

相关产品

Streptavidin (Streptomyces avidinii) Recombinant Protein 丁香通标准抗体
品牌:abnova  货号:P4990  应用:十二烷基硫酸钠聚丙烯酰胺凝胶电泳  价格:¥4980.0 
规格编号:C600432-0001概述:Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.使用说明:This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.Purity > 98 % by SDS-PAGE and HPLC analysesSource Recombinant streptavidin from Streptomyces avidinii, produced in Escherichia coli.Specific Activity > 17 U/mg (one unit binds 1 μg D-biotin at pH 8.9)Formulation Lyophilized in 10 mM potassium phosphate buffer pH 6.5.Endotoxin Less than 0.1 EU/μg of rStreptavidin as determined by LAL method.Reconstitution Dissolve with double dbbic600432
Streptavidin is a tetrameric protein composed of identical subunits. Each subunit binds one biotin molecule with a KD of ~1×10^-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.sciencell1005-01
Concentration:         1 mg/ml, Buffer=0.01M TBS(pH 7.4) with 1% BSA,0.03% Proclin300 and 50% Glycerol.Application Notes:    ELISA=1:200-2000  IHC=1:200-1000     ICC=1:200-1000   WB=1:200-500optimal dilutions/concentrations should be determined by the end user.Storage Instructions:     Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least six weeks at 2-4 °C.Background:Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compaslbse068
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.slbsf068

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