Fig 1. 10 ug of membranes prepared from CHO cells stably expressing ADRA1A receptors were incubated with indicated concentrations of [3H]Prazosin in the absence (total binding) or presence of 1000-fold excess unlabeled Prazosin (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.
Fig 2. 10 ug of membranes prepared from CHO cells stably expressing ADRA1A receptors were incubated with indicated concentrations of Phentolamine in the presence of 1nM [3H]Prazosin. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.