Formulation: 250 µg/mL antibody stored in PBS, pH 7, with 50% glycerol
Storage & Stability: Store product at –200C. Three years from date of shipping
Immunogen: Acetylated KLH conjugates
Purification: The antibody was purified on acetyl-lysine agarose
Specificity: The antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non-acetylated proteins
Scientific Description: DNA transcription can not take place unless DNA is unwound from the nucleosomes. The cell unwinds the DNA by acetylation of lysine residues within the proteins. It has been suggested that acetylation of non-histones proteins (e.g., transcription factors) and histones may be involved. Acetylation of the proteins may result in signal transduction within the chromatic domains.
References: Zeng, L. et al FEBS Lett. 2002, 513, 124-128 (2002) Kim. S. et al Molecular Cell, Volume 23, Issue 4, Pages 607-618