Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
Western blot analysis of (1) HeLa whole cell lysates (2)MDBK whole cell lysates (3) COS-1 whole cell lysates (4) MDCK whole cell lysates with GAPDH Antibody.
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