Pyruvate dehydrogenase (PDH) is the first component enzyme of the pyruvate dehydrogenase complex (PDC). As a key regulator of tricarboxylic acid (TCA) cycle flux, the PDC catalyzes the conversion of pyruvate into acetyl-CoA and regulates the entry of glucose-carbons into the TCA cycle. PDC deficiency is one of the most common neurodegenerative disorders associated with abnormal mitochondrial metabolism. This colorimetric assay is based on pyruvate dehydrogenase-catalyzed oxidation of pyruvate, where the resulting NADH can then convert a nearly colorless probe to a colored product. The intensity of the colored product is proportional to the amount of PDH in the sample, exhibiting maximum absorbance at 440nm.
Product Use
The PDH Assay kit measures the PDH activity of different types of samples, such as tissues and cell lysate. This product is for research purposes only and not for use in animals, humans, or diagnostic procedures.