上海妍琦生物科技有限公司是一家面向生命科学领域,专业经营进口胎牛血清、细胞因子、ELISA试剂盒等实验室试剂、化工产品、实验室消耗品、实验室耗材以及实验室器材。，产品领域：分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学等领域,致力于为中国生命科学研究领域的客户提供最优质的产品、最先进的技术和最专业的服务。主要经营进口试剂品牌有：Roche 、 GE 、 SIGMA 、 abcam 、Thermo、 Peprotech 、 Cayman 、 BioLegend 、 Bio-Rad 、 EnZo 、 CST 、 Life、BD eBioscience STANTCRUZ Millipore RD 等，耗材品牌有：BIOHIT 、 Corning、BRAND 等。
ATP生物发光检测试剂盒, ATP Bioluminescence Assay Kit
11699695001 ,1 kit ( 1,600 assays (microplate), 800 assays (tube))
Application
The ATP Bioluminescence Assay Kit CLS II is optimized for easy use in tube luminometers and microplate-format luminometers. The kit exhibits a constant light signal that is sustained for several minutes. The kit is well suited for kinetic studies and ATP determinations in coupled enzymatic reactions.
For more highly sensitive ATP determinations, use the ATP Bioluminescence Assay Kit HS II.
Contents
Luciferase Reagent, 8 x 10 ml, lyophilized, aliquoted
ATP Standard, 4 x 10 mg, lyophilized (for calibration), aliquoted
Principle
The luciferase from Photinus pyralis (American firefly) catalyzes the following reaction:
ATP + D-luciferin + O 2→ oxyluciferin + PP i + AMP + CO 2 + light.
The quantum yield for this reaction is about 90%. The resulting green light has an emission maximum at 562 nm.
The Michaelis equation has the following form:
light intensity = (V max x C ATP)/(K m + C ATP).
At low ATP concentrations (C ATP<K m), the formula is simplified to light intensity = V max x C ATP/K m.
From this equation, it becomes obvious that the light output is directly proportional to the ATP concentration (C ATP), and is dependent on the amount of luciferase (V max) present in the assay.
Therefore, for maximum sensitivity, the sample ATP must be in a minimum volume, and the luciferase reagent must not be diluted.
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温馨提示：不可用于临床治疗。
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