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ATP生物发光检测试剂盒, ATP Bioluminescence Assay Kit
11699695001 ,1 kit ( 1,600 assays (microplate), 800 assays (tube))
Application
The ATP Bioluminescence Assay Kit CLS II is optimized for easy use in tube luminometers and microplate-format luminometers. The kit exhibits a constant light signal that is sustained for several minutes. The kit is well suited for kinetic studies and ATP determinations in coupled enzymatic reactions.
For more highly sensitive ATP determinations, use the ATP Bioluminescence Assay Kit HS II.
Contents
Luciferase Reagent, 8 x 10 ml, lyophilized, aliquoted
ATP Standard, 4 x 10 mg, lyophilized (for calibration), aliquoted
Principle
The luciferase from Photinus pyralis (American firefly) catalyzes the following reaction:
ATP + D-luciferin + O 2→ oxyluciferin + PP i + AMP + CO 2 + light.
The quantum yield for this reaction is about 90%. The resulting green light has an emission maximum at 562 nm.
The Michaelis equation has the following form:
light intensity = (V max x C ATP)/(K m + C ATP).
At low ATP concentrations (C ATP<K m), the formula is simplified to light intensity = V max x C ATP/K m.
From this equation, it becomes obvious that the light output is directly proportional to the ATP concentration (C ATP), and is dependent on the amount of luciferase (V max) present in the assay.
Therefore, for maximum sensitivity, the sample ATP must be in a minimum volume, and the luciferase reagent must not be diluted.
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