Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Involved in signal transduction through the Wnt pathway. Nuclear beta-catenin it involved in transcriptional regulation by association with transcription factors of the TCF/LEF family.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
参考文献:
[1]"E-cadherin and APC compete for the interaction with beta-catenin and the cytoskeleton."
Huelsken J., Birchmeier W., Behrens J.J. Cell Biol. 127:2061-2069(1994)
图片:
Western blot
All lanes : Catenin beta-1 antibody at at 2 µg/ml
Lane 1 : EC109 whole cell lysate
Lane 2 : 293T whole cell lysate
Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size : 86 kDa
Observed band size: 86 kDa
Additional bands at : 27 kDa. We are unsure as to the identity of this extra band.