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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Mouse
96T
货号 | EK1215 |
---|---|
产品名称 | Mouse uPAR ELISA Kit |
产品描述 | For quantitative detection of mouse uPAR in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, L24-T297 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <5pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | PLAUR |
基因全名 | plasminogen activator, urokinase receptor |
Uniprot ID | P35456 |
指标别名 | CD87|Monocyte activation antigen Mo3|PLAUR|U-PAR|uPAR|UPAR_HUMAN|Urokinase plasminogen activator surface receptor |
背景资料 | The urokinase-type plasminogen activator receptor(uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes.1 The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator.2 UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer.3 PLAUR is located at chromosome 19q13.1-q13.2.1 |
显色时间 | 15-20 分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1215-CAP | 96-well plate precoated with anti- mouse uPAR antibody | 1 |
EK1215-ST | Lyophilized recombinant mouse uPAR standard | 10ng/tube×2 |
EK1215-DA | Biotinylated anti- mouse uPAR antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 102 | 569 | 1345 | 111 | 532 | 1266 |
Standard deviation | 7.95 | 40.39 | 71.28 | 10.21 | 42.02 | 81.02 |
CV(%) | 7.8% | 7.1% | 5.3% | 9.2% | 7.9% | 6.4% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.053 | 0.117 | 0.177 | 0.278 | 0.452 | 0.778 | 1.347 | 1.946 |
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