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发货周期:现货
博士德生物
cell culture supernates and cell lysates.
Rat
96T
货号 | EK0847 |
---|---|
产品名称 | Rat TrkA ELISA Kit |
产品描述 | For quantitative detection of rat TrkA in cell culture supernates and cell lysates. |
免疫原 | NSO, A33-P418 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 78pg/ml-5000pg/ml |
基因名称 | NTRK1 |
基因全名 | neurotrophic tyrosine kinase, receptor, type 1 |
Uniprot ID | P35739 |
指标别名 | High affinity nerve growth factor receptor|MTC|Neurotrophic tyrosine kinase receptor type 1|NTRK1|NTRK1_HUMAN|Oncogene TRK|p140-TrkA|TRK|Trk-A|TRK1|TRK1-transforming tyrosine kinase protein |
背景资料 | Tropomyosin receptor kinase A(TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF(for Brain-Derived Neurotrophic Factor), NT-3(for Neurotrophin-3) and NT-4(for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in keratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 The standard product used in this kit is extracellular part(A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0847-CAP | 96-well plate precoated with anti- rat TrkA antibody | 1 |
EK0847-ST | Lyophilized recombinant rat TrkA standard | 10ng/tube×2 |
EK0847-DA | Biotinylated anti- rat TrkA antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 51 | 251 | 890 | 60 | 259 | 903 |
Standard deviation | 2.24 | 14.06 | 59.96 | 3.54 | 18.6 | 70.4 |
CV(%) | 4.4% | 5.6% | 6.4% | 5.9% | 7.2% | 7.8% |
Concentration(pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.006 | 0.146 | 0.252 | 0.509 | 0.948 | 1.353 | 1.903 | 2.378 |
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