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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Rat
96T
货号 | EK1324 |
---|---|
产品名称 | Rat IL-3 ELISA Kit |
产品描述 | For quantitative detection of rat IL-3 in cell culture supernates and serum. |
免疫原 | E.coli, I27-C169 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 7.8pg/ml-500pg/ml |
基因名称 | IL3 |
基因全名 | interleukin 3 |
Uniprot ID | P04823 |
指标别名 | Burst promoting activity|Colony stimulating factor multiple|Eosinophil colony stimulating factor|Hematopoietic growth factor|IL 3|IL-3|IL3|IL3_HUMAN|Interleukin 3|Interleukin-3|Mast cell growth factor|MCGF|Multi CSF|Multilineage colony stimulating factor|Multipotential colony stimulating factor|Multipotential colony-stimulating factor|P cell stimulating factor|P-cell-stimulating factor|Thy1 inducing factor |
背景资料 | The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1324-CAP | 96-well plate precoated with anti- rat IL-3 antibody | 1 |
EK1324-ST | Lyophilized recombinant rat IL-3 standard | 10ng/tube×2 |
EK1324-DA | Biotinylated anti- rat IL-3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 16 | 91 | 256 | 14 | 84 | 249 |
Standard deviation | 0.8 | 5.36 | 16.64 | 0.77 | 5.37 | 17.43 |
CV(%) | 5% | 5.9% | 6.5% | 5.5% | 6.4% | 7% |
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.011 | 0.059 | 0.096 | 0.184 | 0.299 | 0.767 | 1.478 | 2.267 |
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