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发货周期:现货
博士德生物
CCS/CL/S/P
Mouse
96T
产品名称 | Mouse BDNF ELISA Kit |
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产品描述 | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BDNF |
引用此产品 | Mouse BDNF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK0309) |
验证种属 | Mouse |
免 疫 原 | 标准品表达系统:sf21,H129-R247 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
规 格 | 96 wells/kit |
敏感性 | <4pg/ml 敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。 |
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检测范围 | 31.2pg/ml-2000pg/ml*检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心。 |
样品类型 | cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA, citrate). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。 |
包被抗体 | monoclonal antibody from rat |
检测抗体 | polyclonal antibody from goat |
一般表达浓度 | 以下文章提供了BDNF的表达量的信息: 24604632 |
保存条件 | 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄) |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
- | 96-well plate precoated with anti- mouse BDNF antibody | 1 |
ST0000-10 | lyophilized recombinant mouse BDNF standard | 10ng/tubex2 |
AR1107 | biotinylated anti- mouse BDNF antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | sample diluent buffer | 30ml |
AR1106-2 | antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
- | Adhesive cover | 4 |
1. 96孔读板仪器。
2. 自动洗板机.
3. 移液器和枪头。建议使用多通道移液器/排枪。
4. Eppendorf 管。
5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450ul of purified acetic acid or 700ul of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。基因名 | BDNF |
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蛋白名 | Brain-derived neurotrophic factor |
基因全名 | Brain-derived neurotrophic factor |
蛋白功能 | During development, promotes the survival anddifferentiation of selected neuronal populations of the peripheraland central nervous systems. Participates in axonal growth,pathfinding and in the modulation of dendritic growth andmorphology. Major regulator of synaptic transmission andplasticity at adult synapses in many regions of the CNS. |
Subcellular Localization | Secreted. |
Uniprot ID | BDNF: P21237 |
指标别名 | Brain-derived neurotrophic factor; BDNF; Bdnf; |
相关研究领域 | neuroscience|neurology process|growth and development|neurotrophins|neurodegenerative disease|huntington""s disease|alzheimer""s disease|cardiovascular|atherosclerosis|diabetes associated|thrombosis|metabolism|types of disease|diabetes|obesity |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 105 | 436 | 1217 | 136 | 524 | 1279 |
Standard deviation | 3.57 | 16.13 | 49.90 | 9.24 | 38.25 | 94.65 |
CV(%) | 3.4 | 3.7 | 4.1 | 6.8 | 7.3 | 7.4 |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.063 | 0.095 | 0.133 | 0.216 | 0.358 | 0.720 | 1.405 | 2.298 |
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