Aliquots of ∼100 ng of purified total RNA in a 100 µL volume were concentrated using a SpeedVac into tubes with RNAconcentrator or into empty tubes (SpeedVac), and also using ethanol precipitation (EtOH ppt.). Concentrated samples were resuspended in 10 µL water and used as input template for reverse transcription PCR (RT-PCR) for amplification of human Hsp90 amplicon (2.3 kb). Results indicate more abundant amplification from RNA templates concentrated with RNAconcentrator as compared to conventional methods.