This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml."
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
WB - Quality tested IHC - Reported in the literature
Application References
1. Murray G, et al. 1998. Gut. 43:791. (IHC) 2. Murray G, et al. 1998. J. Pathol. 185:256. (IHC) 3. Duncan ME, et. al. 1998. Eur. J. Biochem. 258:37. (IHC)
Description
Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade substances within the extracellular matrix. The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. Other MMPs, plasmins as well as other factors activate MMPs. MMPs are thought to play an important role in tissue remodeling associated with various physiological and pathological processes. MMP9 degrades of proteins in the extracellular matrix and activates growth factors like proTGFb and proTNFa. MMP-9 contributes to the invasion and metastasis of various human malignancies. Clone F11P2C3 has been shown to be useful for western blotting and immunohistochemistry of human MMP9.