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DNA extractor kit(生物制药,血清)

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Wako(和光纯药工业株式会社)
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成立时间:1922
入驻丁香通年限:4年
商家等级:金牌客户
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联系人:代理商宝柏

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产品详情

品牌: Wako
货号: 295-50201
保存条件: 冷藏
供应商: Wako(和光纯药工业株式会社)
规格: 50tests
生物制剂中残留DNA的提取。

传统的方式是采用蛋白酶-SDS的方法提取DNA,虽然能够获得高品质的DNA,但是后续需要用酚-氯仿进行处理,而酚-氯仿属于危险化学品,对人体有一定危害。

该试剂盒采用碘化钠法,能获得高质量和高回收率的DNA,抽提过程不需要酚-氯仿。

碘化钠代替酚-氯仿,溶解蛋白和脂类。糖原作为载体,异丙醇沉淀DNA。

只需将试剂加入离心管内即可,不像Qiagen的DNA抽提试剂盒采用柱吸附法。

产品回收率10pg左右。

文献报道的回收率是80%~90%。

Molecular Biology-DNA Extraction Kits. Detects and quantitates contaminant DNA in serum and residual DNA in biopharmaceuticals. Employs a new extraction procedure for DNA purification from a single tube. This procedure, using Sodium Iodide (NaI) as a chaotropic agent, realizes DNA isolation of both high quality and high recovery from biological fluids without the use of phenol or chloroform. Complex and laborious manipulations are avoided when using this kit. In addition, this kit has a modified application for use with Molecular Devices' Threshold(TM) System.
PRINCIPLE of DNA EXTRACTION: A high concentration of chaotropic reagent, NaI, and an anionic detergent participate in solubilization of the proteins and lipids contained in biological samples. After addition of isopropanol to the mixture, nucleic acids are co-precipitated with polysaccharide glycogen as a carrier, while other components remain soluble in the solution phase.
Ref.: Ishizawa, M., Kobayashi, T. and Matsuura, S., ''Simple procedure of DNA Isolation from Human Serum'', Nucleic Acids Res., 19, 5792 (1991).
温馨提示:不可用于临床治疗。
资料下载: 295-50201.pdf 附件下载 (下载6 次)

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生物制剂中残留DNA的提取。传统的方式是采用蛋白酶-SDS的方法提取DNA,虽然能够获得高品质的DNA,但是后续需要用酚-氯仿进行处理,而酚-氯仿属于危险化学品,对人体有一定危害。该试剂盒采用碘化钠法,能获得高质量和高回收率的DNA,抽提过程不需要酚-氯仿。碘化钠代替酚-氯仿,溶解蛋白和脂类。糖原作为载体,异丙醇沉淀DNA。只需将试剂加入离心管内即可,不像Qiagen的DNA抽提试剂盒采用柱吸附法。产品回收率10pg左右。文献报道的回收率是80%~90%。Molecular Biology-DNA Extraction Kits. Detects and quantitates contaminant DNA in serum and residual DNA in biopharmaceuticals. Employs a new extraction procedure for DNA purification from a single tube. This procedure, using Sodium Iodide (NaI) as a chaotropic agent, realizes DNA isolation of both high quality and high recovery from biological fluids without the use of phenol or chloroform. Complex and laborious manipulations are avoided when using this kit. In addition, this kit has a modified application for use with Molecular Devices' Threshold(TM) System.PRINCIPLE of DNA EXTRACTION: A high concentration of chaotropic reagent, NaI, and an anionic detergent participate in solubilization of the proteins and lipids contained in biological sampwako295-50201
DNA extraction for 8-OHdG assayfor DNA extraction from human & animal parenchyma tissueDNA Extractor(r) TIS Kit is specially designed for the extraction of DNA from human and animal parenchyma tissue. The base of this kit is a procedure using Sodium Iodide (NaI) as a chaotropic agent, which allows for a safe extraction without hazardous phenol/chloroform. The procedure using Sodium Iodide is an extraction procedure which causes less oxidation of DNA. Additionally, the DNA Extractor(r) TIS Kit is able to inhibit the oxidation of DNA by using an Oxidation Inhibitor. It is the most useful DNA extraction kit for the assay of 8-OHdG (8-Hydroxy-2'-deoxyguanosine), which is a marker of oxidative stress.[Features] Useful for detection and determination of 8-OHdG, a marker of oxidative stress. Achieve inhibition of the further DNA oxidation by using an oxidation inhibitor Suitable for a wide range of applications, which require DNA extraction from human and animal parenchyma tisswako296-67701
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  DNA Extractor Kit DNA提取试剂盒产品名称:DNA  Extractor? KitDNA提取试剂盒产品编号:295-50201     4OO_ 811 _ 9081 I368I6I966Z 包装:50Tests说明:  DNA Extractor Kit DNA提取试剂盒purification from a single tube. This procedure, using Sodium Iodide (NaI) as a chaotropic agent, realizes DNA isolation of both high quality and high recovery from biological fluids without the use of phenol or chloroform. Complex and laborious manipulations are avoided when using this kit. In addition, this kit has a modified application for use with Molecular Devices' Threshold(TM) System. PRINCIPLE of DNA EXTRACTION: A high concentration of chaotropic reagent, NaI, and an anionic detergent participate in solubilization of the proteins and lipids contained in biological samples. After addition of isopropanol to the mixture, nucleic acids are co-precipitated with polysaccharide glycogen as a carrier, while other components remain soluble in the solution phase. Ref.: Ishizawa, M., Kobayashi, T. and Matsuura, S., ''Simplziqibio295-50201 (50 tests)

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