The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
test sizes: lot-specific; µg size: 0.5 mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
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Applications
ICFC - Quality tested
Application Notes
ELISA or ELISPOT Capture1-3,7: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Neutralization1-3,5,6: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.
Application References
1. Abrams J, et al. 1992. Immunol. Rev. 127:5. 2. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20. 3. Gaines Das R, et al. 1993. J. Immunol. Methods 160:147. 4. Enriquez J, et al. 2002. Adv. Perit Dial. 18:177. 6. Zou JP, et al. 1999. J. Immunol. 162:4882. 7. Wyant TL, et al. 1999. Infect. Immun. 67:1338. 8. Lesmeister MJ, et al. 2005. Reprod. Biol. Endocrinol. 3:74. 9. Terasaka Y, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:2441. PubMed
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Description
IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.