重组人 VEGF121 / VEGF-A 蛋白(产品说明)
蛋白名称:Human VEGF121 / VEGF-A Protein
蛋白构建:A DNA sequence encoding the human VEGF 121 isoform (P15692-9) (Met1-Arg147) was expressed.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1.Measured by its binding ability in a functional ELISA.2.Immobilized human VEGF121(Cat:10008-HNAH) at 10μg/mL (100μL/well) can bind human VEGFR2-Fc(Cat:10012-H02H), the EC50 of human VEGFR2-Fc is 5-50 ng/mL.3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC).The ED50 for this effect is typically 3-15 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 27
蛋白分子量:The recombinant human VEGF consists of 121 amino acids and has a predicted molecular mass of 14 kDa. The apparent molecular mass of the human VEGF 121 is approximately 19.9 and 17.0 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:P15692-9
蛋白氨基酸序列:Met1-Arg147
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.