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- 文献和实验
- 技术资料
- 抗体名:
Mouse Anti-GFP monoclonal antibody
- 抗体英文名:
Mouse Anti-GFP monoclonal antibody
- 抗原:
GFP
- 应用范围:
ELISA;WB;IP
- 宿主:
mouse
- 标记物:
non-conjugated
- 是否单克隆:
单克隆
- 保存条件:
hipped at 4°C Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze
- 形态:
liquid
- 亚型:
IgG2a
- 免疫原:
Recombinant GFP Protein
- 规格:
50ug/100ug
| 规格: | 50ug | 产品价格: | ¥720.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥1200.0 |
- 纯化方式:
>95%,Protein G purified - 标记方式:
Non-conjugated - 保存缓冲液:
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 - 产品提供形式:
liquid - 储存条件:
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. - 图片:

WB: Mouse Anti-GFP monoclonal antibody at 0.1μg/ml
Line 1:HEK-293 cell lysate
Line 2:GFP transfected HEK-293 cell lysate
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 30kd
Observed band size : 30kd
IP:Result of anti-GFP-tag Monoclonal antibody
Line 1:Control IgG
Line 2:Precipitateing GFP-tagged fusion protein
Line 3:GFP Transfected HEK-293 cells lysate
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 30kd
Observed band size : 30kd
- 别名:
CFP, eGFP, eYFP, GFP, GFP tag, YFP - 引用文献:
1. The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility, Gomez-H L, et al,Plos Genetics,2019 2. Microscopic analysis of colonization of Colletotrichum abscissum in citrus tissues , Daiani CristinaSavi,microbiological research,2019
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文献和实验| 1. The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility, Gomez-H L, et al,Plos Genetics,2019 |
| 2. Microscopic analysis of colonization of Colletotrichum abscissum in citrus tissues , Daiani CristinaSavi,microbiological research,2019 |
【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
对照,2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R](货号#E022030),其免疫原是KLH偶联的水母GFP蛋白的N末端多肽,由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致,因此从免疫原上来说,GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是,EarthOx对于该单克隆抗体还进行了明确的验证试验,试验也表明该Anti-GFP Tag Monoclonal
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
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