A rapid, simple, reliable and accurate method for the measurement of total sulphite (sulfite) and free sulphite in wine, beverages, foodstuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for manual, autoanalyser and microplate formats.
INTRODUCTION:
Sulphur dioxide (SO2) is used widely as an additive in various forms,
most commonly as sulphites (or sulfites), in the wine, beverage
and food industries where it acts primarily as an antimicrobial and
antioxidant preservative.
During wine making sulphites are used as an essential additive, usually
at post-malolactic fermentation, in the control of contamination
by Brettanomyces during aging and to also protect the wine against
detrimental “oxidative and enzymatic browning”. SO2 is only active
as an antimicrobial and antioxidant preservative in the unbound
“free” form. Given that SO2 becomes “inactive” when it binds the
colour pigments of wine, and with legal restrictions on SO2 levels in
wine, it has become valuable to wine producers to measure both the
Free SO2 (FSO2) and Total SO2 (TSO2). In addition to this, due to
the increased awareness of the adverse effects of sulphites and the
prevalence of sulphite intolerance in some individuals, sulphite levels
in foods and drinks are strictly regulated by various governing bodies
and therefore there is a requirement for accurate determination of
the level of sulphites in foods, beverages and wines.
This kit (K-SULPH) is suitable for the specific measurement of both
“Total Sulphite” and “Free Sulphite” especially in wines, beverages,
foodstuffs and other materials.
KITS:
Kits suitable for performing 40 assays of each test in manual format
(or 400 assays of each test in auto-analyser format) are available from
Megazyme. The kits contain the full assay method plus:
Bottle 1: Total Sulphite Reagent 1 (40 mL)
Contains sodium azide (0.05 % w/v) as a preservative.
Stable for > 18 months at room temperature.
Bottle 2: Total Sulphite Reagent 2 (20 mL)
Contains sodium azide (0.05 % w/v) as a preservative.
Stable for > 18 months at room temperature.
Bottle 3: Free Sulphite Reagent 1 (40 mL)
Stable for > 18 months at room temperature.
Bottle 4: Free Sulphite Reagent 2 (20 mL)
Stable for > 18 months at room temperature.
Bottle 5: Sulphite Standard
Sodium Sulphite (5 g).
Stable for > 5 years at room temperature.