A simple, reliable and accurate method for the determination of ethanol in beverages and foodstuffs.
INTRODUCTION:
Ethanol is ubiquitous in its natural occurrence, and thus its quantitative
determination is not only important in the manufacture of intoxicating
wines, beers and spirits, but also for low-alcohol and non-alcoholic
beverages, fruit juices and a range of other foodstuffs, including
chocolates, sweets, jam, honey, vinegar and dairy products. A large
range of non-foods also contain significant quantities of ethanol, such as
cosmetics and pharmaceuticals.
KITS:
Kits suitable for performing 60 assays in manual format (or 600
assays in auto-analyser format or 600 assays in microplate format) are
available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (15 mL, pH 9.0) plus sodium azide
(0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: NAD+.
Stable for > 5 years at -20°C.
Bottle 3: Aldehyde dehydrogenase solution (3.25 mL).
Stable for > 2 years at -20°C.
3
Bottle 4: Alcohol dehydrogenase suspension (1.3 mL).
Stable for > 2 years at 4°C.
Bottle 5: Ethanol standard solution (5 mL, 5 mg/mL).
Stable in a well sealed container (as supplied) for > 2
years at 4°C.