AssayMax Rat Haptoglobulin ELISA Kit 大鼠结合珠蛋白Hpt酶联免疫试剂盒
Introduction Haptoglobulin (Hpt) is a plasma protein with hemoglobin-binding capacity and a plasma glycoprotein that forms a stable complex with hemoglobin to aid the recycling of heme iron. It is a well-known marker of hemolysis (1). High haptoglobulin level in plasma was associated with an increased cardiovascular risk in obese men (2), inflammation (3), atherosclerosis (4), and systemic sclerosis (5).
Principle of the Assay The AssayMax Rat Haptoglobulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of rat haptoglobulin in urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures haptoglobulin in less than 4 hours. A polyclonal antibody specific for rat haptoglobulin has been pre-coated onto a microplate. Haptoglobulin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for rat haptoglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.