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    Mouse Complement Factor D ELISA Kit(小鼠补体因子D酶联免疫试剂盒)

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    Mouse Complement Factor D ELISA Kit(小鼠补体因子D酶联免疫试剂盒)

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      • 货号:

        EMF7701-1

      • 样本:

        50 μl

      • 应用:

        血清、血浆、细胞培养上清液、尿液

      • 检测方法:

        ELISA

      • 检测限:

        0.03 ng/ml

      • 供应商:

        AssayPro

      • 数量:

        大量

      • 规格:

        96 wells

      AssayMax Mouse Complement Factor D ELISA Kit
      小鼠补体因子D酶联免疫试剂盒

      Introduction
      Complement Factor D (FD), also known as adipsin, is a serine protease secreted by adipocytes into the bloodstream and a component of the alternative complement pathway. It is a 24 kDa single chain glycoprotein with 222 amino acids and has a high level of expression in fat (1-3). FD cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment. Human FD plays a major role in humoral suppression of infectious agents. The deficiency of FD increases susceptibility to infections and their recurrence (4).
       
      Principle of the Assay
      The AssayMax Mouse Complement Factor D (FD) ELISA kit is designed for detection of mouse FD in plasma, serum, urine, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures mouse FD in less than 4 hours. A polyclonal antibody specific for mouse FD has been pre-coated onto a 96-well microplate with removable strips. Mouse FD in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for mouse FD, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
       

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