AssayMax Human Complement C8 ELISA Kit 补体C8酶联免疫试剂盒
Introduction Complement Component 8 (C8) is a 150-kDa complex composed of three genetically distinct subunits: C8α (64 kDa), C8β (64 kDa), and C8γ (22 kDa). C8α and C8β are highly homologous to each other and to C6, C7 and C9, and contain a common membrane attack complex/perforin (MACPF) domain. C8γ has a lipocalin fold and shares no homology with any other complement protein (1). C8 plays a central role in membrane attack complex MAC assembly by coordinating the interaction with complement proteins C5b-7 and the pore-forming protein C9 on pathogen membranes. It is also the first component to penetrate the lipid bilayer (2-3). C8 deficiency exhibits an increased susceptibility to Neisseria meningitidis infections and recurrent meningococcal disease (4).
Principal of the Assay The AssayMax Human Complement C8 ELISA kit is designed for detection of C8 in human plasma, serum, saliva, milk, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C8 in less than 4 hours. A polyclonal antibody specific for C8 has been pre-coated onto a microplate. C8 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C8, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.