AssayMax Human Complement C6 ELISA Kit 补体C6酶联免疫试剂盒
Introduction Human Complement Component 6 (C6) is a single-chain glycoprotein consisting of 913 amino acid residues with a molecular mass of about 102 kDa (1). C6 is a part of the lytic membrane attack complex during complement activation. Cleavage of C5 into C5a and C5b by C5 convertase triggers binding of plasma C6 to C5b. Once the C5b-6 complex forms, C7, C8, and C9 add sequentially to create a transmembrane channel structure (2). Complete deficiency of C6 (C6Q0) leads to an increased susceptibility to Neisseria meningitidis infections and recurrent meningococcal disease (3). In animal models, genetic C6 deficiency accelerates axonal regeneration (4) and reduces atherosclerosis (5).
Principle of the Assay The AssayMax Human Complement C6 ELISA kit is designed for detection of C6 in human plasma, serum, saliva, urine, milk, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C6 in less than 4 hours. A polyclonal antibody specific for C6 has been pre-coated onto a microplate. C6 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C6, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.