Colonic Epithelial Cell Medium (CoEpiCM) is a complete medium designed for optimal growth of normal
human colonic epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential
amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals.
The medium is serum-free. It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an
incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively)
to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation
and growth of normal human colonic epithelial cells in vitro.
Components
CoEpiCM consists of 500 ml of basal medium, 5 ml of colonic epithelial cell growth supplement (CoEpiCGS,
Cat. No. 2952) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503). Note: Due to BPE in the
growth supplement, formation of lipoproteins can cause precipitates to be present; the color may vary with
different lots.
Product Use
CoEpiCM is for research use only. It is not approved for human or animal use, or for application in in vitro
diagnostic procedures.
Storage
Store the basal medium at 4°C, the CoEpiCGS and the P/S solution at -20°C. Protect from light.