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- 文献和实验
- 技术资料
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
rp302947-100μg/rp302947-10μg/rp302947-1mg/rp302947-50μg
| 规格: | rp302947-100μg | 产品价格: | ¥299.90 |
|---|---|---|---|
| 规格: | rp302947-10μg | 产品价格: | ¥99.90 |
| 规格: | rp302947-1mg | 产品价格: | ¥1099.90 |
| 规格: | rp302947-50μg | 产品价格: | ¥199.90 |
基本描述
产品名称:Recombinant Human EGF Protein
规格或纯度:无动物源,无载体,生物活性,ActiBioPure™,高性能,≥95%(SDS-PAGE),expressed in E. coli; 见COA
应用:SDS-PAGE, Cell Culture, Functional Studies
生物活性:Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.67 ng/mL.
氨基酸:971-1023 aa
无载体:Yes
无动物源:Yes
Accession #:
预测分子量:6.2 kDa
SDS-PAGE:6.0 kDa, under reducing conditions; 6.0 & 11.8 kDa, under non-reducing conditions.
级别:无动物源, 无载体, 生物活性, ActiBioPure™, 高性能
产品规格参数
储存缓冲液:Lyophilized from PBS, pH 7.4
浓度:expressed in E. coli; 见COA
复溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:-20℃保存 12个月;复溶后建议分装,避免反复冻融。
功能和特点
Accession #:
生物活性:Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.67 ng/mL.
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文献和实验Production and Characterization of Recombinant Human and Murine TNF
and the identity of the final products can be checked by SDS-PAGE, ELISA, Western blot, analytical gel-filtration chromatography, mass spectrometry, and lipopolysaccharide assay. The biological activity of both human and murine TNF is assessed by in vitro cytolytic
Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
) Serum‐free medium of choice Recombinant FGF‐basic (Gibco #PHG0021L) Recombinant EGF (Gibco #PHG0311
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