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- 文献和实验
- 技术资料
- 抗体名:
CD4 (mouse specific) Rat Monoclonal Antibody 抗体
- 抗体英文名:
CD4 (mouse specific) Rat Monoclonal Antibody
- 靶点:
CD4 (mouse specific)
- 应用范围:
FC, IF
- 宿主:
Rat
- 适应物种:
Mouse
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1294280
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
详询
- 亚型:
Rat IgG2a
- 免疫原:
详询
- 规格:
25 ul/100 ul
| 规格: | 25 ul | 产品价格: | ¥3276.0 |
|---|---|---|---|
| 规格: | 100 ul | 产品价格: | ¥5031.0 |
产品子类型: Primary Antibody
描述: Rat Monoclonal antibody to CD4 (mouse specific)
克隆号: BTO6X7
应用稀释比例: Immuno fluorescence 1:100-1:200, Flow Cytometry 1:50-1:100
免疫原: Synthetic peptide / Extracellular domain corresponding to Mouse CD4
防腐剂: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
研究领域: Immunology & Inflammation
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.


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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
me why this works!) before cloning out for variants. b) You can screen by ELISA or with mAb coupled RBC using mouse monoclonal antibodies against the Y3 derived Kappa 1a (RG11/15.5) and the specific DA Kappa 1b (G9/1.3), or the appropriate heavy chain (eg NORIG
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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