Caspase-9 cleaved Asp315 Rabbit Polyclonal Antibody 抗体,orb1294352,Biorbyt产品图

Caspase-9 cleaved Asp315 Rabbi

t Polyclonal Antibody 抗体,orb1294352,Biorbyt
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  • ¥3042 - 4563
  • Biorbyt已认证
  • orb1294352
  • 英国
  • 2026年07月31日
  • IF, IHC, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Caspase-9 cleaved Asp315 Rabbit Polyclonal Antibody 抗体

    • 抗体英文名

      Caspase-9 cleaved Asp315 Rabbit Polyclonal Antibody

    • 靶点

      Caspase-9 cleaved Asp315

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb1294352

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 亚型

      Rabbit-IgG

    • 免疫原

      详询

    • 规格

      25 ul/100 ul

    规格:25 ul产品价格:¥3042.0
    规格:100 ul产品价格:¥4563.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Rabbit polyclonal antibody to Caspase 9 cleaved Asp315

    应用稀释比例: Western Blot 1:500, Immunofluorescence 1:300-1:400, Immunohistochemistry (Paraffin) 1:100-1:300

    免疫原: Synthetic peptide corresponding to residues surrounding Asp315 of human Caspase 9

    防腐剂: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

    研究领域: Cell Biology

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    All lanes: Anti-Caspase 9 cleaved Asp315 antibody - at 1/500 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294352 overnight at 4°C.

    产品细节图片2
    All lanes: Anti-Caspase 9 cleaved Asp315 antibody - at 1/500 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294352 overnight at 4°C.

    产品细节图片3
    Immunofluorescence: cells were fixed with 4% Fixative for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294352 anti-Caspase 9 cleaved Asp315 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

    产品细节图片4
    Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Caspase 9 cleaved Asp315 with orb1294352 at 1/200.

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    图标文献和实验
    相关实验
    • Genomic Libraries

      of icosahedral phage particle which contains the viral genome:   During replication, the phage DNA is produced in a concatameric form, which is cleaved by appropriate endonucleases to allow packaging of a single genome within the phage

    • Antigen Design Sera Purification Tech Sheet

      carrier protein. It is a good choice as a second carrier protein when verifying whether antibodies are specific for the peptide alone and not the carrier. Rabbit Serum Albumin (RSA) may be used when the antibody response to the carrier protein must be kept

    • Antigen Design Sera Purification Tech Sheet

      alone and not the carrier. Rabbit Serum Albumin (RSA) may be used when the antibody response to the carrier protein must be kept to a minimum. Rabbits immunized with RSA conjugate are less likely to raise antibodies to the carrier, as the RSA is recognized as "self

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