- ￥5499
- Biorbyt
- orb1291670
- 英国
- 2026年07月31日
- FC, IHC, WB
- Rabbit
- Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
Tim 2/Timd2 Rabbit Polyclonal Antibody 抗体
- 抗体英文名：
Tim 2/Timd2 Rabbit Polyclonal Antibody
- 靶点：
T-cell immunoglobulin and mucin domain-containing protein 2
- 浓度：
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- 应用范围：
FC, IHC, WB
- 宿主：
Rabbit
- 适应物种：
Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1291670
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Lyophilized
- 亚型：
Rabbit IgG
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
产品子类型: Primary Antibody
描述: Anti-Tim 2/Timd2 Antibody. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Mouse, Rat.
别名: Timd2; T-cell immunoglobulin and mucin domain-containing protein 2; TIMD-2; T-cell immunoglobulin mucin receptor 2; TIM-2
应用稀释比例: Western blot, 0.25-0.5 μg/ml, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat Immunofluorescence, 5 μg/ml, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Mouse
免疫原: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Tim 2/Timd2, which shares 73.9% amino acid (aa) sequence identity with rat Timd2.
交叉反应: No cross-reactivity with other proteins.
分子量: 70 kDa
防腐剂: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
纯化方法: Immunogen affinity purified.
复溶/重构: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
研究领域: Immunology & Inflammation
UniProt ID: Q8R183
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: Anti-Tim 2/Timd2 Antibody. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Mouse, Rat.
别名: Timd2; T-cell immunoglobulin and mucin domain-containing protein 2; TIMD-2; T-cell immunoglobulin mucin receptor 2; TIM-2
应用稀释比例: Western blot, 0.25-0.5 μg/ml, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat Immunofluorescence, 5 μg/ml, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Mouse
免疫原: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Tim 2/Timd2, which shares 73.9% amino acid (aa) sequence identity with rat Timd2.
交叉反应: No cross-reactivity with other proteins.
分子量: 70 kDa
防腐剂: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
纯化方法: Immunogen affinity purified.
复溶/重构: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
研究领域: Immunology & Inflammation
UniProt ID: Q8R183
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Flow Cytometry analysis of HEPA1-6 cells using anti-Tim 2/Timd2 antibody. Overlay histogram showing HEPA1-6 cells (Blue line). The cells were fixed with 4% Fixative and blocked with 10% normal goat serum. And then incubated with rabbit anti-Tim 2/Timd2 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of Tim 2/Timd2 using anti-Tim 2/Timd2 antibody. Tim 2/Timd2 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-Tim 2/Timd2 Antibody overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of Tim 2/Timd2 using anti-Tim 2/Timd2 antibody. Tim 2/Timd2 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Tim 2/Timd2 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Tim 2/Timd2 using anti-Tim 2/Timd2 antibody. Tim 2/Timd2 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Tim 2/Timd2 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Western blot analysis of Tim 2/Timd2 using anti-Tim 2/Timd2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tim 2/Timd2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Tim 2/Timd2 at approximately 70 kDa. The expected band size for Tim 2/Timd2 is at 34 kDa.
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Tim 2/Timd2 Rabbit Polyclonal Antibody 抗体，orb1291670，Biorbyt
￥5499
