gamma Synuclein/SNCG Rabbit Polyclonal Antibody 抗体，orb1291673，Biorbyt产品图

gamma Synuclein/SNCG Rabbit Po

lyclonal Antibody 抗体，orb1291673，Biorbyt
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  • ￥5499
  • Biorbyt已认证
  • orb1291673
  • 英国
  • 2026年07月31日
  • FC, WB
  • Rabbit
  • Human
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      gamma Synuclein/SNCG Rabbit Polyclonal Antibody 抗体

    • 抗体英文名：

      gamma Synuclein/SNCG Rabbit Polyclonal Antibody

    • 靶点：

      Gamma-synuclein

    • 浓度：

      Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

    • 应用范围：

      FC, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human

    • 保质期：

      12 months from date of receipt.

    • 抗原来源：

      详询

    • 目录编号：

      orb1291673

    • 级别：

      科研

    • 库存：

      99

    • 供应商：

      Biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 保存条件：

      详询

    • 形态：

      Lyophilized

    • 亚型：

      Rabbit IgG

    • 免疫原：

      详询

    • 规格：

      100 ug

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Anti-gamma Synuclein/SNCG Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

    别名: Laminin subunit gamma-1; Laminin B2 chain; Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; S-laminin subunit gamma; S-LAM gamma; Lamc1; Lamb-2, Lamc-1

    应用稀释比例: Western blot, 0.25-0.5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human

    免疫原: A synthetic peptide corresponding to a sequence at the C-terminus of human gamma Synuclein/SNCG, which shares 53.8% amino acid (aa) sequence identity with rat SNCG.

    交叉反应: No cross-reactivity with other proteins.

    分子量: 17 kDa

    防腐剂: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    纯化方法: Immunogen affinity purified.

    复溶/重构: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

    研究领域: Cell Biology, Neuroscience, Signal Transduction

    UniProt ID: O76070

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Flow Cytometry analysis of Hela cells using anti-gamma Synuclein/SNCG antibody. Overlay histogram showing Hela cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% Fixative and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-gamma Synuclein/SNCG Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

    产品细节图片2
    Western blot analysis of gamma Synuclein/SNCG using anti-gamma Synuclein/SNCG antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-gamma Synuclein/SNCG antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for gamma Synuclein/SNCG at approximately 17 kDa. The expected band size for gamma Synuclein/SNCG is at 13 kDa.

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