- ￥13338
- Biorbyt
- orb1251982
- 英国
- 2026年07月31日
- FC, IF, IHC-P, WB
- Mouse
- Human, Mouse
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- 文献和实验
- 技术资料
- 抗体名：
NCL Antibody 抗体
- 抗体英文名：
NCL Antibody
- 靶点：
NCL
- 浓度：
0.2 mg/mL
- 应用范围：
FC, IF, IHC-P, WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1251982
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 形态：
Liquid
- 亚型：
IgG1
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
产品子类型: Primary Antibody
描述: NCL Antibody
克隆号: NPC23-2
别名: Nucleolin, Protein C23, NCL
应用说明: IF: 1-2 µg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlWestern blot: 1-2 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the Nucleolin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: Recombinant human protein was used as the immunogen for the Nucleolin antibody.
防腐剂: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
UniProt ID: P19338
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: NCL Antibody
克隆号: NPC23-2
别名: Nucleolin, Protein C23, NCL
应用说明: IF: 1-2 µg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlWestern blot: 1-2 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the Nucleolin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: Recombinant human protein was used as the immunogen for the Nucleolin antibody.
防腐剂: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
UniProt ID: P19338
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
IHC testing of FFPE human tonsil with Nucleolin antibody (clone NPC23-2).
IHC testing of FFPE human ovarian carcinoma with Nucleolin antibody (clone NPC23-2).
Flow cytometry testing of human 293 cells and Alexa Fluor 488-labeled Nucleolin antibody (green).
Western blot testing of Nucleolin antibody and A431 lysate. Predicted molecular weight ~77 kDa.
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文献和实验相关实验
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
sapiens replication protein A2, 32kDa (RPA2), mRNA 537 NM_004356 Homo sapiens CD81 antigen (target of antiproliferative antibody 1) (CD81), mRNA 3638 NM_001743 Homo sapiens calmodulin 2 (phosphorylase kinase, delta) (CALM2), mRNA 4675 NM_004231
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NCL Antibody 抗体，orb1251982，Biorbyt
￥13338
