- ￥13338
- Biorbyt
- orb1252433
- 英国
- 2026年07月31日
- FC, IF, IHC-P
- Mouse
- Human
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- 文献和实验
- 技术资料
- 抗体名：
CD34 Antibody 抗体
- 抗体英文名：
CD34 Antibody
- 靶点：
CD34
- 浓度：
0.2 mg/mL
- 应用范围：
FC, IF, IHC-P
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1252433
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 形态：
Liquid
- 亚型：
IgG1, kappa
- 免疫原：
详询
- 规格：
100 ug
产品子类型: Primary Antibody
描述: CD34 Antibody
克隆号: HPCA1/763
别名: Hematopoietic progenitor cell antigen CD34, CD34, CD34
应用说明: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.25-0.5 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the CD34 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: Recombinant full-length human protein was used as the immunogen for the CD34 antibody.
防腐剂: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
研究领域: Immunology & Inflammation
UniProt ID: P28906
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验for 10 min at 400 x g to remove excess DETACHaBEAD. 14) Resuspend the cells in Buffer or Culture Medium. 15) The isolated cells are pure, viable and free from antibody bound to the surface, and may be used in any downstream application
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
antibody and resuspend in PBS/2% FCS. 10. Separate the CD34+ cells as follows: i. Wash a MACS cell separation column with 200 µL of PBS/2% FCS. ii. Add the labeled cells to the column and place
Construction and Purification of Adhesion Molecule Immunoglobulin Chimeric Proteins
for a given cell-surface molecule. Particularly useful in this respect have been soluble forms of proteins created by fusing the extracellular domains of the protein of interest to the Fc part of an antibody molecule (1 ). The resulting dimeric proteins
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