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- 文献和实验
- 技术资料
- 抗体名:
Mitochondria Antibody [MTC02] 抗体
- 抗体英文名:
Mitochondria Antibody [MTC02]
- 浓度:
0.2 mg/mL
- 应用范围:
IF, IHC, WB
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1252656
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
详询
- 形态:
Liquid
- 亚型:
IgG1, kappa
- 免疫原:
详询
- 规格:
100 ug
产品子类型: Primary Antibody
描述: Mitochondria Antibody [MTC02]
克隆号: MTC02
应用说明: Western blot: 1-2 µg/mLIF: 0.5-1 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Mitochondria antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: A semi-purified mitochondria preparation was used as the immunogen for the Mitochondria antibody.
防腐剂: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
特异性: Does not react with mouse and rat
研究领域: Cell Biology
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.



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文献和实验, which was detectable after 3 min of clivorine treatment. The results from the same membrane reprobed with total-p38 antibody demonstrated that the total amount of p38 MAP Kinase in L-02 cells was not changed. We also tested the effect of another pyrrolizidine alkaloid
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
in mitochondria. O2 •– generation by ETC also induces oxidative damage with protein radical formation. Immunospin-trapping with anti-DMPO antibody and subsequent mass spectrometry are used to define the specific site of oxidative damage, indicating cysteine-206
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