- ￥10764
- Biorbyt
- orb1249087
- 英国
- 2026年07月31日
- ELISA, FC, IF, IHC, WB
- Goat
- Human
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- 技术资料
- 抗体名：
PLA2G4A Antibody 抗体
- 抗体英文名：
PLA2G4A Antibody
- 靶点：
PLA2G4A
- 浓度：
500 ug/mL
- 应用范围：
ELISA, FC, IF, IHC, WB
- 宿主：
Goat
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1249087
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Liquid
- 免疫原：
详询
- 规格：
0.1 mg
类别: Antibodies
产品子类型: Primary Antibody
描述: PLA2G4A Antibody
别名: PLA2G4A, phospholipase A2, group IVA (cytosolic, calcium-dependent), MGC126350, PLA2G4, cPLA2-alpha, calcium-dependent phospholipid-binding protein, cytosolic phospholipase A2, group IVA, lysophospholipase, phosphatidylcholine 2-acylhydrolase
应用说明: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80-85kDa band observed in lysates of cell line A549 (calculated MW of 85.2kDa according to NP_077734.1). Preliminary experiments also showed an 85- 90kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:0.3-1µg/mL. Primary incubation 1 hour at room temperature.Preliminary testing was unsuccessful on Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 3.75µg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10µg/mL.
免疫原: The immunogen for this antibody is: C-QEKTFRQQRKEHIR
预测反应性: Mouse, Rat
防腐剂: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
纯化方法: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
研究领域: Metabolism Research
UniProt ID: P47712
NCBI 参考序列: NP_077734.1
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: PLA2G4A Antibody
别名: PLA2G4A, phospholipase A2, group IVA (cytosolic, calcium-dependent), MGC126350, PLA2G4, cPLA2-alpha, calcium-dependent phospholipid-binding protein, cytosolic phospholipase A2, group IVA, lysophospholipase, phosphatidylcholine 2-acylhydrolase
应用说明: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80-85kDa band observed in lysates of cell line A549 (calculated MW of 85.2kDa according to NP_077734.1). Preliminary experiments also showed an 85- 90kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:0.3-1µg/mL. Primary incubation 1 hour at room temperature.Preliminary testing was unsuccessful on Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 3.75µg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10µg/mL.
免疫原: The immunogen for this antibody is: C-QEKTFRQQRKEHIR
预测反应性: Mouse, Rat
防腐剂: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
纯化方法: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
研究领域: Metabolism Research
UniProt ID: P47712
NCBI 参考序列: NP_077734.1
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
orb1249087 (1 ug/ml) staining of A549 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1249087 Immunofluorescence analysis of Fixative fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.
orb1249087 Flow cytometric analysis of Fixative fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).
orb1249087 (3.75 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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PLA2G4A Antibody 抗体，orb1249087，Biorbyt
￥10764
