- ￥5499
- Biorbyt
- orb547486
- 英国
- 2026年07月27日
- IHC, WB
- Mouse
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
Collagen III/COL3A1 Mouse Monoclonal Antibody 抗体
- 抗体英文名：
Collagen III/COL3A1 Mouse Monoclonal Antibody
- 靶点：
Collagen alpha-1
- 浓度：
500 µg/ml
- 应用范围：
IHC, WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb547486
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 形态：
Lyophilized
- 亚型：
Mouse IgG2b
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
产品子类型: Primary Antibody
描述: Anti-Collagen III/COL3A1 Antibody (monoclonal, B1J1). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
克隆号: B1J1
别名: COL3A1; Collagen alpha 1(III) chain; Collagen Type III; EDS4A
应用稀释比例: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
免疫原: E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222- E1455).
交叉反应: No cross-reactivity with other proteins.
分子量: 180-190 kDa
防腐剂: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cell Biology, Musculoskeletal & Connective Tissue Research, Protein Biochemistry, Signal Transduction, Stem Cell & Developmental Biology
UniProt ID: P02461
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: Anti-Collagen III/COL3A1 Antibody (monoclonal, B1J1). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
克隆号: B1J1
别名: COL3A1; Collagen alpha 1(III) chain; Collagen Type III; EDS4A
应用稀释比例: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
免疫原: E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222- E1455).
交叉反应: No cross-reactivity with other proteins.
分子量: 180-190 kDa
防腐剂: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cell Biology, Musculoskeletal & Connective Tissue Research, Protein Biochemistry, Signal Transduction, Stem Cell & Developmental Biology
UniProt ID: P02461
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody. Collagen III/COL3A1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-Collagen III/COL3A1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody. Collagen III/COL3A1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-Collagen III/COL3A1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody. Collagen III/COL3A1 was detected in paraffin-embedded section of human lung cancerr tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-Collagen III/COL3A1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody. Collagen III/COL3A1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-Collagen III/COL3A1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody. Collagen III/COL3A1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-Collagen III/COL3A1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody. Collagen III/COL3A1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-Collagen III/COL3A1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: Hela whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Collagen III/COL3A1 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Collagen III/COL3A1 at approximately 180-190KD. The expected band size for Collagen III/COL3A1 is at 180-190KD.
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