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- CAS号：
120241-79-4
- 规格：
1mg/1mLx10mM(inDMSO)/5mg/10mg/25mg/50mg/100mg/500mg
| 规格： | 1mg | 产品价格： | ￥190.0 |
|---|---|---|---|
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥384.0 |
| 规格： | 5mg | 产品价格： | ￥384.0 |
| 规格： | 10mg | 产品价格： | ￥658.0 |
| 规格： | 25mg | 产品价格： | ￥1192.0 |
| 规格： | 50mg | 产品价格： | ￥1992.0 |
| 规格： | 100mg | 产品价格： | ￥3016.0 |
| 规格： | 500mg | 产品价格： | ￥6704.0 |
Product Introduction
Bioactivity
| 名称 | 3-TYP |
| 描述 | 3-TYP (3-(1H-1,2,3-triazol-4-yl) pyridine) is a selective SIRT3 inhibitor. |
| 细胞实验 | Cell viability is analyzed using Cell Counting Kit-8. 1×104 cells are inoculated into 96-well plates. After being treated, 90 μL of medium and 10 μL of CCK-8 solution are added to each well. The cells are then incubated at 37°C for 2 h. After incubation, the absorption at 450 nm is measured using an Infinite? M200 Microplate Reader. The results are expressed as a percentage of the control. The cell death is also evaluated using the trypan blue assay. HepG2 cells are plated in the 6-well plates (5×105 cells per well) and incubated for 24 h. After being treated with Cd or melatonin, the cells are detached with 300 μL trypsin-EDTA solution. The mixture of detached cells is centrifugated at 300 g for 5 min. Then, the residue is combined with 800 μL trypan blue solution and dispersed. After 3 min staining, cells are counted using an automated cell counter. The dead cells are stained with the blue color. Cell mortality (%) is expressed as percentage of the dead cell number/the total cell number. |
| 动物实验 | 3-TYP is formulated in 1% ethanol.Male C57BL/6 mice are anesthetized with 2% isoflurane, and myocardial ischemia is produced by temporarily exteriorizing the heart via a left thoracic incision and placing a 6-0 silk suture slipknot around the left anterior descending coronary artery. After 30 minutes of myocardial ischemia, the slipknot is released, and the myocardium is reperfused for 3 hour (for western blot analysis and oxidative stress measurement) or 24 hour (for cardiac function, apoptotic index and infarct size determination). Sham-operated mice undergo the same surgical procedures except the suture placed under the left coronary artery is not tied. Ten minutes before reperfusion, mice are randomized to receive either vehicle (1% ethanol) or melatonin (20 mg/kg) by intraperitoneal injection. C57BL/6 mice are randomly divided into the following groups: (i) Sham group: mice underwent the sham operation and are treated with vehicle (1% ethanol); (ii) Mel group: mice are treated with melatonin (20 mg/kg via intraperitoneal injection); (iii) IR+V group: mice underwent the MI/R operation and are treated with vehicle (1% ethanol); (iv) IR+Mel group: mice underwent the MI/R operation and are treated with melatonin (20 mg/kg via intraperitoneal injection 10 minutes before reperfusion); (v) IR+Mel+3-TYP group: mice are pretreated with 3-TYP (3-TYP is intraperitoneally injected at a dose of 50 mg/kg every 2 days for a total of three doses prior to the MI/R surgery), subjected to the MI/R operation, and treated with melatonin (20 mg/kg via intraperitoneal injection 10 minutes before reperfusion); and (vi) IR+3-TYP group: mice are pretreated with 3-TYP and then subjected to the MI/R operation. |
| 体外活性 | 3-TYP显著减弱了在Cd暴露的HepG2细胞中，褪黑激素诱导的去乙酰化SOD2表达和SOD2活性的增加。3-TYP抑制了褪黑激素增强的SIRT3活性，但并不影响SIRT3蛋白表达。3-TYP预处理逆转了褪黑激素对镉(Cd)引起的线粒体来源的O2•−产生和自噬细胞死亡的保护效果。 |
| 体内活性 | 3-TYP显著降低SIRT3活性，增加了SOD2的乙酰化水平，但不影响SIRT3的表达。3-TYP通过减少LVEF和LVFS，降低了褪黑激素的心脏保护效应，此效应在24小时再灌注后显著。另外，与IR+Mel组相比，3-TYP增加了梗塞面积、血清LDH水平和凋亡比率。3-TYP（50 mg/kg, i.p.）与Sham组相比，对LVEF、LVFS、梗塞面积、血清LDH水平、凋亡及氧化应激的影响不明显。此外，与Sham组相比，3-TYP对gp91phox、Nrf2、NQO 1、Bax、Bcl-2、Caspase-3及活化Caspase-3的表达水平影响甚微。 |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 5 mg/mL (34.21 mM), Sonication is recommended. H2O : 5 mg/mL (34.21 mM), Sonication is recommended. Ethanol : 28 mg/mL (191.58 mM), Sonication is recommended. DMSO : 231 mg/mL (1580.57 mM), Sonication is recommended. |
| 关键字 | Sirtuin | SIRT3 | SIRT2 | SIRT1 | Inhibitor | inhibit | 3-TYP | 3TYP | 3 TYP |
| 相关产品 | Selisistat | Fisetin | Indoximod | Palmatine | Nicotinamide riboside | Nicotinamide | Dihydrocoumarin | Nicotinamide riboside malate | Epacadostat | SRT 2104 | β-Lapachone | SIRT5 inhibitor 8 |
| 相关库 | Inhibitor Library | Bioactive Compound Library | Anti-Cancer Compound Library | Bioactive Compounds Library Max | Mitochondria-Targeted Compound Library | Chromatin Modification Compound Library | Anti-Aging Compound Library | Glutamine Metabolism Compound Library | Antioxidant Compound Library | DNA Damage & Repair Compound Library | Target-Focused Phenotypic Screening Library | Reprogramming Compound Library |
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相关实验
网络 第十九章 杂环化合物和生物碱 杂环化合物在自然界分布很广，其数量几乎占已知有机化合物的三分之一，用途也很多。许多重要的物质如叶绿素、血红素、核酸以及临床应用的一些有显著疗效的天然药物和合成药物等，都含有杂环化合物的结构。生物碱多是中草药的有效成分，绝大多数是含氮的杂环化合物。本章内容与医学关系密切，具有重要意义。
分子中具有高能键的化合物。在生物体内主要有上式（Ⅰ）和（Ⅱ）两种结构的高能化合物。其作用为贮藏能量（例如 ATP、肌酸磷酸）和作为产生 ATP源的中间代谢产物（例如磷酸烯醇丙酮酸）以及合成反应的中间物质（例如酰基辅酶 A），在蛋白质的结构（ conformation）变化周期的第一阶段上（例如钠钾 ATP酶的羧基磷酸中间体）也具有重要作用。多数高等化合物具有磷酸基，但也有例外。
由不同种元素组成的纯净物叫做化合物。化合物一般有固定的组成。化合物的组成一般可用化学式表示。化合物具有确定的物理性质和化学性质，不同于其组成元素的性质。化合物中的元素不能用简单的机械方法或物理方法分开，而必须用化学方法才能分离。
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化合物 3-TYP【120241-79-4】
￥190 - 6704
