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- 详细信息
- 文献和实验
- 技术资料
- CAS号:
115-33-3
- 规格:
1mLx10mM(inDMSO)/2mg/5mg/10mg/25mg/50mg/100mg/200mg
| 规格: | 1mLx10mM(inDMSO) | 产品价格: | ¥153.0 |
|---|---|---|---|
| 规格: | 2mg | 产品价格: | ¥102.0 |
| 规格: | 5mg | 产品价格: | ¥138.0 |
| 规格: | 10mg | 产品价格: | ¥214.0 |
| 规格: | 25mg | 产品价格: | ¥330.0 |
| 规格: | 50mg | 产品价格: | ¥438.0 |
| 规格: | 100mg | 产品价格: | ¥638.0 |
| 规格: | 200mg | 产品价格: | ¥936.0 |
Product Introduction
Bioactivity
| 名称 | Oxyphenisatin acetate |
| 描述 | Oxyphenisatin acetate inhibits the growth of the breast cancer cell lines MCF7, T47D, HS578T, and MDA-MB-468. |
| 细胞实验 | Total RNA was isolated from MCF7 cells treated with 10 μmol/L OXY for 24 h and the microarray procedure performed as described previously using the GeneChip Human U133 plus 2.0 array . Pairwise analysis was performed on control versus treated arrays using a fivefold change cutoff, <0.01 adjusted P-value, GC-RMA normalization with Benjamini–Hochberg false discovery estimation[1]. |
| 体外活性 | 在雌激素受体(ER)阳性的MCF7和T47D细胞中,OXY诱导TNFα表达和TNFR1降解,表明这些细胞系中发生了自分泌受体介导的凋亡。最后,在MCF7异种移植模型中,腹腔内给予OXY抑制了肿瘤生长,伴随eIF2α的磷酸化和TNFR1的降解。OXY引发的多方面细胞饥饿反应,最终诱导程序性细胞死亡[1]。 |
| 存储条件 | Store at low temperature,Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | DMSO : 150 mg/mL (373.68 mM), Sonication is recommended. |
| 关键字 | Oxyphenisatin acetate | Oxyphenisatin | Inhibitor | inhibit | Autophagy |
| 相关产品 | Guanidine hydrochloride | Naringin | Enzalutamide | Aceglutamide | Alginic acid | Cysteamine hydrochloride | Hemin | Hydroxychloroquine | Sildenafil citrate | Stavudine | Tamoxifen | Paeonol |
| 相关库 | Anti-Breast Cancer Compound Library | Anti-Cancer Active Compound Library | Bioactive Compound Library | Anti-Cancer Compound Library | Bioactive Compounds Library Max | Autophagy Compound Library | Anti-Aging Compound Library | NO PAINS Compound Library | Endoplasmic Reticulum Stress Compound Library |
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文献和实验0.25 110 90 128 64 139 101 45 0.25 0.30 134 78 115 63 119 90 45 122 97 71 32 0.30 0.35 125 99 58 109 85 47 109 88 67 34 90 72 52 24 0.35 0.40 115 97 77 45 101 85 66 37 117 84 68 51 26 101 70 55
【共享】microRNA protocols (english edition)
........................................ 33 4. Recognition and Cleavage of Primary MicroRNA Transcripts Yan Zeng and Bryan R. Cullen ............................................. 49 5. Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference
我先说我有的质粒和菌株:PPIC9K,PPIC9,PPICZahphaA,B,C 以及一个改造的PPIC9K载体,EcoRI和BamHI双酶切,C末端引入了一个6histag 菌株:GS115,KM71,X33,以及十几个空载体对照菌株 我的酵母表达Protocol: >10ug质粒用Sal线性化,加1/10体积的3M NaAC PH5.2和3倍体积的乙醇沉淀 70%乙醇洗一遍,20ul ddH2O重溶 按照公司手册做电转化感受态,一般OD
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