3-((4-bromobenzyl)thio)-6-meth

【资源】一个pQE30系统表达蛋白纯化案例

reached 1.0, isopropyl-1-thio-β-d-galactopyranoside was added to a final concentration of 1 mM. After 5 h of induction, the cells were harvested by centrifugation at 4,000 × g for 20 min. 样品处理 For the purification of protein from 2 liters of culture

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

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2'-deprotection/annealing reaction. After deprotection and annealing, the RNA pellet is in 400 µl buffer. To ethanol precipitate, adjust the solution to 0.3 M NaCl by addition of 26 µl 5 M NaCl. Finally, add 1500 µl absolute ethanol and vortex. After 1 to 2 h