相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
IL-1 alpha/IL-1A, Cynomolgus
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
100μg/500μg
| 规格: | 100μg | 产品价格: | ¥3800.0 |
|---|---|---|---|
| 规格: | 500μg | 产品价格: | ¥12600.0 |
| Species | Cynomolgus | |
| Protein Construction |
|
|
| Purity | > 95% as determined by BisTris PAGE | |
| Endotoxin Level | Less than 1EU per μg by the LAL method. | |
| Expression System | HEK293 | |
| Theoretical Molecular Weight | 18.13 kDa | |
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 26-33 kDa based on Bis-Tris PAGE result. | |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | |
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
| Target Background | The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases. |
| Synonyms | Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1; IL-1 ALPHA; IL1α; IL-1A; IL1 |
产品特点:
1. 【食蟹猴源天然序列】采用食蟹猴源 IL-1α/IL-1A 天然氨基酸序列,无突变,与天然蛋白结构完全一致,可特异性结合 IL-1 受体2. 【高活性高特异性】可高效诱导炎症反应,ED₅₀ < 100 ng/mL(细胞活化实验),可被 IL-1α 特异性抗体识别,适用于抗体筛选、炎症免疫机制研究3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.2 EU/μg(LAL 法),适配免疫细胞培养、体内动物实验等敏感场景需求4. 【易纯化检测】可通过亲和层析快速纯化,也可通过 IL-1α 特异性抗体进行检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 IL-1α 蛋白结构与功能研究、炎症与免疫调控机制研究、抗体制备与筛选、药物靶点验证、细胞培养添加剂等
IL-1α,IL-1A, 食蟹猴源,食蟹猴,重组蛋白,重组,高活性,高纯度,低内毒素,炎症免疫,细胞培养,免疫细胞,科研实验,哺乳动物细胞表达 Cynomolgus,IL-1α,IL-1A,Recombinant protein,High activity,High purity,Low endotoxin,Inflammatory immunity,Cell culture,Immune cell,Mammalian expression
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验加入不同稀释度的IL-1α或IL-1β,100μl/孔, 同时设1%FCS RPMI 1640对照 ↓ 5%CO,37℃培养18~24小时 ↓ 按终稀释度为1∶10~20转入另一块96孔板中
和 caspase-1 p10 裂解被强烈抑制(图 2F)。实时共焦显微镜分析显示,EST12 导致腹腔巨噬细胞呈现典型的细胞焦亡形态:质膜破裂,多个泡状突起,细胞核内呈 “煎蛋” 状(图 2G)。H37RvΔEST12 感染 WT BMDMs 和 H37Rv 感染 RACK1−/−BMDMs 的细胞凋亡和 IL-1β分泌无区别(图 2H)。BCG-EST12 和 BCG 感染 RACK1−/−BMDMs 的细胞毒性和 IL-1β分泌无明显区别(图 2I)。M. smeg 和 M. smeg-EST12 实验
仪以检测波长570nm,参考波长630nm,分别测定各孔OD值。测定应在酸化异丙醇加入后lh内完成。 【结果分析】 (1) 每孔OD值应为OD570 nm-OD630 nm,再减去培养液对照OD值,最终每孔OD值应取3复孔的平均值; (2) 以log2 [稀释度]为X(横座标),各稀释度对应的OD值为Y(纵座标),在普通坐标纸上,分别绘制出IL-1标准品与待测样品两条回归曲线(如图)。 IL-1标准品与待测样品两条回归
技术资料暂无技术资料 索取技术资料








