- ￥1176 - 9828
- abinScience
- France
- HA050023
- 2026年04月27日
- WB: 1:500-1:2000
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 形态：
Liquid
- 保存条件：
-20°C
- 克隆性：
单克隆
- 保质期：
twelve months
- 级别：
一抗
- 供应商：
武汉研升生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB: 1:500-1:2000
- 抗体英文名：
Anti-Synaptotagmin-12 Antibody (N277/7)
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1176.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1956.0 |
| 规格： | 1mg | 产品价格： | ￥9828.0 |
| 产品名 | Anti-Synaptotagmin-12 Antibody (N277/7) |
| 货号 | HA050023 |
| 宿主 | Mouse |
| 克隆类型 | Monoclonal |
| 克隆号 | N277/7 |
| 靶标 | Synaptotagmin-12, SytXII, SYT12, Synaptotagmin XII |
| 同种型 | IgG2a, kappa |
| 应用 | IHC, WB |
| 应用范围 | WB: 1:500-1:2000 |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 纯度 | >95% as determined by SDS-PAGE. |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 说明 | For research use only. |
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文献和实验Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
IMMUNOSCREENING OF WITH ANTIBODY PROBES
in 10mM IPTG (MW=238.31). Briefly blot the excess solution from the filters, and wrap them in cellophane. These should be moist and stored at 4°C. We typically adsorb serum with five 150 mm Petri dishes of lysed E. coli to reduce anti-E. coli antibody
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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