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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
单克隆
- 适应物种:
Phleum pratense (Common timothy)
- 保质期:
twelve months
- 级别:
一抗
- 供应商:
武汉研升生物科技有限公司
- 宿主:
Human
- 应用范围:
ELISA: 1:2000-1:20000
- 抗体英文名:
Anti-PHLPV/Allergen Phl p V Antibody (SAA0721)
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1338.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2288.0 |
| 规格: | 1mg | 产品价格: | ¥11148.0 |
| 产品名 | Anti-PHLPV/Allergen Phl p V Antibody (SAA0721) |
| 货号 | PR120143 |
| 宿主 | Human |
| 种属反应性 | Phleum pratense (Common timothy) |
| 克隆类型 | Monoclonal |
| 克隆号 | SAA0721 |
| Accession号 | Q40960 |
| 靶标 | PHLPV, Pollen allergen Phl p 5, Allergen Phl p V |
| 同种型 | IgG1 |
| 应用 | ELISA |
| 应用范围 | ELISA: 1:2000-1:20000 |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 纯度 | >95% as determined by SDS-PAGE. |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 说明 | For research use only. |

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文献和实验7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
Immunization Protocol for Monoclonal Antibody Production
followed by i.v. or i.p. boost Saline/PBS i.v. 69 fusion 3d after i.v. boost or 4d after i.p. boost � i.v. 上一篇:Polyclonal Antibody Production 下一篇:The production
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