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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
单克隆
- 适应物种:
Human
- 保质期:
twelve months
- 级别:
一抗
- 供应商:
武汉研升生物科技有限公司
- 宿主:
Mouse
- 应用范围:
WB: 1:500-1:2000
- 抗体英文名:
Anti-Phospho-Vimentin (pS83) Antibody (MO82)
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1338.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2288.0 |
| 规格: | 1mg | 产品价格: | ¥11148.0 |
| 产品名 | Anti-Phospho-Vimentin (pS83) Antibody (MO82) |
| 货号 | HY550033 |
| 宿主 | Mouse |
| 种属反应性 | Human |
| 克隆类型 | Monoclonal |
| 克隆号 | MO82 |
| Accession号 | P08670 |
| 靶标 | Vimentin, VIM |
| 同种型 | IgG2a, kappa |
| 应用 | IHC, IP, WB |
| 应用范围 | WB: 1:500-1:2000 |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 纯度 | >95% as determined by SDS-PAGE. |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 说明 | For research use only. |

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文献和实验3.0 ml ethanolamine before use(10) 1X Buffer A0.1 M NaOAc 0.82 g NaOAc0.5 M NaCl 2.92 g NaClpH to 4.0 and bring up to 100 ml2. Procedure(1) Pour a 5 ml affigel blue column (biorad) and wash with 50 ml Prewash to prep the column (when first used
Antibody Purification This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography. Solutions Affigel Blue Prewash 0.1 M acetic acid 5.7 ml glacial acetic acid
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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