人蛋白激酶C结合蛋白NELL1(NELL1)酶联免疫吸附检测
试剂盒【Human NELL1 (Protein kinase C-binding protein NELL1) ELISA Kit】- ￥1960 - 2800
- ELK Biotechnology
- 31.25-2000 pg/mL
- 13.5 pg/mL
- 武汉
- ELK9986
- 2026年05月01日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
31.25-2000 pg/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, plasma, tissue homogenates and other biological fluids
- 灵敏度：
13.5 pg/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 人蛋白激酶C结合蛋白NELL1(NELL1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human NELL1 (Protein kinase C-binding protein NELL1) ELISA Kit |
| 别名 | NEL-like protein 1;Nel-related protein 1;NRP1 |
| 货号 | ELK9986 |
| 反应种属 | Human |
| Q92832 | Q92832 |
| 检测类型 | Sandwich |
| 灵敏度 | 13.5 pg/mL |
| 标准品 | 2000 pg/mL |
| 检测范围 | 31.25-2000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NELL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NELL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NELL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NELL1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Neuroscience |
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文献和实验Analysis of Protein Kinase C Function
Protein kinase C is a key element in signal transduction, cell regulation, and tumor promotion, influencing cellular responses to many stimuli at a variety of levels, including receptors, transduction systems, ion channels, and membrane pumps
Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
PKC系统(protein kinase C system，PKC system)
( diacylglycerol, DAG)和1,4,5-三磷酸肌醇(IP3)。IP3动员细胞内钙库释放Ca2 到细胞质中与钙调蛋白结合，随后参与一系列的反应；而DAG在Ca2 的协同下激活蛋白激酶C(protein kinase C，PKC)，然后通过蛋白激酶C引起级联反应，进行细胞的应答, 故此将该系统称为PKC系统,或称为IP3、DAG、Ca2 信号通路。
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