- ￥1820 - 2600
- ELK Biotechnology
- 0.32-20 ng/mL
- 0.132 ng/mL
- 武汉
- ELK9971
- 2026年04月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
Tissue homogenates and other biological fluids.
- 灵敏度：
0.132 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 小鼠含黄素单加氧酶3(FMO3)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse FMO3(Flavin Containing Monooxygenase 3) ELISA Kit |
| 别名 | Dimethylaniline oxidase 3; Fetal hepatic flavin-containing monooxygenase 3 |
| 货号 | ELK9971 |
| 反应种属 | Mouse |
| P97501 | P97501 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.132 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FMO3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FMO3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FMO3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FMO3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |
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文献和实验Benzydamine N-Oxygenation as a Measure of Flavin-Containing Monooxygenase Activity
Benzydamine is a nonsteroidal anti-inflammatory drug that undergoes flavin-containing monooxygenase (FMO)-dependent metabolism to a stable N -oxide. This metabolite can be quantified with high specificity and sensitivity by using a simple
Prochiral Sulfoxidation as a Probe for Flavin-Containing Monooxygenases
Asymmetric aryl alkyl sulfides (R-S-R′) are metabolized by flavin-containing monooxygenase (FMO) and cytochrome P450 enzymes to enantiomerically enriched sulfoxide products (R-SO-R′) that are readily analyzed with a host of commercially
抗原（或抗体）的浓度。操作过程中颇为费时费力的是各步之间的洗板过程。虽然目前有自动化洗板仪器商供，但普通实验室通常并不置备。在小规模的实验（如只有一块酶联板）中，手工向各反应孔中加入洗液（含0.05% Tween的PBS缓冲液）尚可行。但如有较大规模（如5-10块酶联板）实验，即使使用多道移液器吸加洗液也很易使人疲劳。本文以使用商业试剂盒检测人IL6和自行检测小鼠血清中抗体为例，介绍ELISA的一种简便操作方法。人IL6检测试剂盒为eBioscience公司（San Diego, CA, USA
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