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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Cattle
- 样本:
serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
0.069 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 牛皮肤桥蛋白(DPT)酶联免疫吸附检测试剂盒 |
| 英文名称 | Cattle DPT(Dermatopontin) ELISA Kit |
| 别名 | TRAMP; Tyrosine-rich acidic matrix protein |
| 货号 | ELK9962 |
| 反应种属 | Cattle |
| 检测类型 | Sandwich |
| 灵敏度 | 0.069 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle DPT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle DPT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle DPT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle DPT in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Infection immunity; |

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文献和实验相关实验
牛白介素 6(IL6)酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定牛血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL6 含量。试剂盒内容试剂名称数量试剂名称数量96孔板(预包被)196孔板覆膜2标准品2标准品稀释液1×20mL检测溶液A1×120μL检测稀释液A1×12mL检测溶液B1×120μL检测稀释液B1×12mLTMB底物1×9mL终止液1×6mL浓洗涤液(30×)1×20mL使用说明书1需自备的设备及试剂1.450±10nm 滤光
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牛皮肤桥蛋白(DPT)酶联免疫吸附检测试剂盒【Cattle DPT(Dermatopontin) ELISA Kit】
¥1960 - 2800





