大鼠磷脂酰肌醇4,5-二磷酸3-激酶催化亚基α(PIK3Ca
)酶联免疫吸附检测试剂盒【Rat PIK3Ca(Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide) ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 31.25-2000 pg/mL
- 11.3 pg/mL
- 武汉
- ELK9935
- 2026年04月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
31.25-2000 pg/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
11.3 pg/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 大鼠磷脂酰肌醇4,5-二磷酸3-激酶催化亚基α(PIK3Ca)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat PIK3Ca(Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide) ELISA Kit |
| 别名 | phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; CCM4; CLAPO; CLOVE; CWS5; MCAP; MCM; MCMTC; PI3K; PI3K-alpha; p110-alpha |
| 货号 | ELK9935 |
| 反应种属 | Rat |
| 检测类型 | Sandwich |
| 灵敏度 | 11.3 pg/mL |
| 标准品 | 2000 pg/mL |
| 检测范围 | 31.25-2000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PIK3Ca. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIK3Ca. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PIK3Ca, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIK3Ca in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |
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文献和实验Phosphoinositide (PI) 3-Kinase Assays
The regulation of phosphoinositide (PI) 3-kinase activities has been linked to many normal and disease-related processes, including cell survival, cell growth and proliferation, cell differentiation, cell motility, and intracellular vesicle
Measurement of Phosphoinositide 3-Kinase Activity
The term “PI 3-kinase” is now applied to a growing family of proteins that are able to convert phosphatidylinositol (PtdIns), PtdIns(4)P and PtdIns(4,5)P 2 into PtdIns(3)P, PtdIns(3,4)P 2 and PtdIns(3,4,5)P 3
Measurement of Phosphoinositide 3-Kinase Activation by Interleukins
Phosphoinositide 3-kinases (PI 3-kinases) are an evolutionarily conserved family of lipid kinases that have attracted much attention over the past 10 years or so (reviewed in ref. 1 ). Three PI 3-kinase classes
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